Take the first possible option, whether it's direct or a transfer; you never know when the system will break down.
Don't sit next to anyone who looks messy, figuratively or literally; that stuff can rub off on you.
Always face forward.
They're called personal hygiene and personal affairs for a reason; if you should be doing it in the bathroom or the bedroom, you shouldn't be doing in front of strangers.
Stand up for the elderly, the pregnant, and the disadvantaged. If you're feeling generous, women in high heels and nurses at the end of the day.
Handle your business; your inability to hang on is no excuse for knocking others over.
You only get one seat; if you have that much stuff, you need to stand or find another way to get where you're going.
Wash yourself and your clothes regularly.
Sometimes there will be delays and problems down the line; recognize there is nothing you can do about them and comport yourself accordingly.
Stand on the right, walk on the left.